What is the formula that LabCollector uses for Tm in Sequences module? - LabCollector

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Two standard approximation calculations are used.

For sequences less than 14 nucleotides the formula is:
Tm= (wA+xT) * 2 + (yG+zC) * 4

For sequences longer than 13 nucleotides, the equation used is
Tm= 64.9 +41*(yG+zC-16.4)/(wA+xT+yG+zC)

where w,x,y,z are the number of the bases A,T,G,C in the sequence, respectively.