What is the formula that LabCollector uses for Tm in Sequences module?
Two standard approximation calculations are used. For sequences less than 14 nucleotides the formula is: Tm= (wA+xT) * 2 + (yG+zC) * 4 For sequences longer than 13 nucleotides, the equation used is Tm= 64.9 +41*(yG+zC-16.4)/(wA+xT+yG+zC) where w,x,y,z are the … Continued
